Vitamin D-Binding Protein antibody (AA 164-213)

Catalog No. ABIN6741530

Product Details anti-Vitamin D-Binding Protein Antibody

Target: Vitamin D-Binding Protein (GC)

Binding Specificity: AA 164-213

Reactivity: Human, Mouse, Rat, Monkey, Dog, Cow, Horse, Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vitamin D-Binding Protein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human DBP

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa164-213 of human DBP (Q10586, NP_001343). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Dog, Bovine, Horse, Pig (100%), Elephant (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Vitamin D-Binding Protein

Target: Vitamin D-Binding Protein (GC)

Alternative Name: D-Binding Protein / DBP (GC Products)

Synonyms: DBP antibody, DBP/GC antibody, GRD3 antibody, VDBG antibody, VDBP antibody, zgc:110389 antibody, zgc:92753 antibody, GC antibody, Gc antibody, DBP02 antibody, VDB antibody, Vdbp antibody, VTDB antibody, gc antibody, GC, vitamin D binding protein antibody, D-box binding PAR bZIP transcription factor antibody, D site albumin promoter binding protein antibody, group-specific component (vitamin D binding protein) antibody, group specific component antibody, group-specific component (vitamin D binding protein) S homeolog antibody, GC antibody, Dbp antibody, gc antibody, Gc antibody, gc.S antibody

Background: Name/Gene ID: DBP

Synonyms: DBP, D-binding protein, DABP, Albumin D box-binding protein, TaxREB302, D site-binding protein

Gene ID: 1628

NCBI Accession: NP_001343

UniProt: Q10586

Pathways: Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process