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FHL1 antibody (AA 51-100)

FHL1 Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, Sheep, Guinea Pig, Hamster WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6741535
  • Target See all FHL1 Antibodies
    FHL1 (Four and A Half LIM Domains 1 (FHL1))
    Binding Specificity
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 51-100
    Reactivity
    • 44
    • 15
    • 13
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, Sheep, Guinea Pig, Hamster
    Host
    • 34
    • 6
    • 5
    Rabbit
    Clonality
    • 38
    • 7
    Polyclonal
    Conjugate
    • 34
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FHL1 antibody is un-conjugated
    Application
    • 34
    • 17
    • 11
    • 8
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human FHL1
    Predicted Reactivity
    Percent identity by BLAST analysis: Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Pig, Guinea pig (100%) Horse (92%) Human, Xenopus (91%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa51-100 of human FHL1 (Q6IB30, NP_001440). Percent identity by BLAST analysis: Human, Chimpanzee, Galago, Mouse, Rat, Sheep, Hamster, Dog, Bovine, Rabbit, Pig, Guinea pig (100%), Horse, Platypus, Xenopus (92%), Gorilla, Orangutan, Gibbon, Monkey, Marmoset (91%), Elephant, Turkey, Zebra finch (83%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product FHL1 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    FHL1 (Four and A Half LIM Domains 1 (FHL1))
    Alternative Name
    SLIM / FHL1 (FHL1 Products)
    Synonyms
    fhl1 antibody, MGC89165 antibody, FHL1 antibody, DKFZp468O1927 antibody, fhl antibody, fhla antibody, zgc:92025 antibody, FHL-1 antibody, FHL1A antibody, FHL1B antibody, FLH1A antibody, KYOT antibody, SLIM antibody, SLIM-1 antibody, SLIM1 antibody, SLIMMER antibody, XMPMA antibody, KyoT antibody, Fhl1 antibody, four and a half LIM domains 1 antibody, four and a half LIM domains 1a antibody, Four and a half LIM domains protein 1 antibody, four and a half LIM domains 1 S homeolog antibody, fhl1 antibody, FHL1 antibody, fhl1a antibody, fhl1.S antibody, Fhl1 antibody
    Background
    Name/Gene ID: FHL1

    Synonyms: FHL1, BA535K18.1, FHL1B, FHL1A, FLH1A, Four and a half LIM domains 1, FHL-1, KYOT, Skeletal muscle LIM-protein 1, SLIM-1, Skeletal muscle LIM-protein1, SLIMMER, XMPMA, KYO-T, LIM protein SLIMMER, SLIM, SLIM1
    Gene ID
    2273
    NCBI Accession
    NP_001440
    UniProt
    Q13642
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