HMGN1 antibody (AA 36-85)

Catalog No. ABIN6741544

Product Details anti-HMGN1 Antibody

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Binding Specificity: AA 36-85

Reactivity: Human, Mouse, Rat, Cow, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human HMGN1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine (100%) Rabbit (92%) Rat (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human HMGN1 (P05114, NP_004956). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Baboon, Monkey, Bovine, Horse, Armadillo (100%), Gibbon, Rabbit (92%), Opossum (91%), Platypus (86%), Marmoset, Pig (85%), Rat (84%), Mouse (76%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HMGN1

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Alternative Name: HMGN1 (HMGN1 Products)

Synonyms: HMG14 antibody, hmg14 antibody, hmgn1 antibody, hmgn1b antibody, HMG-14 antibody, Hmg14 antibody, high mobility group nucleosome binding domain 1 antibody, high mobility group nucleosome binding domain 1 S homeolog antibody, high mobility group nucleosomal binding domain 1 antibody, non-histone chromosomal protein HMG-14 antibody, HMGN1 antibody, hmgn1.S antibody, hmgn1 antibody, Hmgn1 antibody, LOC785479 antibody

Background: Name/Gene ID: HMGN1

Synonyms: HMGN1, HMG14

Gene ID: 3150

NCBI Accession: NP_004956

UniProt: P05114

Pathways: Chromatin Binding