BACH2 antibody (C-Term)

Catalog No. ABIN6741581

Product Details anti-BACH2 Antibody

Target: BACH2 (BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 2 (BACH2))

Binding Specificity: C-Term

Reactivity: Human, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BACH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human BACH2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Horse, Guinea pig (92%) Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human BACH2 (Q9BYV9, NP_068585). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset (100%), Gibbon, Mouse, Rat, Horse, Guinea pig (92%), Galago, Elephant, Rabbit (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BACH2

Target: BACH2 (BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 2 (BACH2))

Alternative Name: BACH2 (BACH2 Products)

Synonyms: E030004N02Rik antibody, Bach2 antibody, BTBD25 antibody, bach2 antibody, si:dkeyp-4f12.1 antibody, si:dkeyp-4f12.2 antibody, BTB and CNC homology, basic leucine zipper transcription factor 2 antibody, BTB domain and CNC homolog 2 antibody, BTB and CNC homology 1, basic leucine zipper transcription factor 2b antibody, Bach2 antibody, BACH2 antibody, bach2b antibody, bach2 antibody

Background: Name/Gene ID: BACH2

Synonyms: BACH2, BTBD25, BTB and CNC homolog 2

Gene ID: 60468

NCBI Accession: NP_068585

UniProt: Q9BYV9