TRIM8 antibody (AA 36-85)

Catalog No. ABIN6741584

Product Details anti-TRIM8 Antibody

Target: TRIM8 (Tripartite Motif Containing 8 (TRIM8))

Binding Specificity: AA 36-85

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, Zebrafish (Danio rerio), Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TRIM8 / GERP

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Horse, Chicken, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human TRIM8 (Q9BZR9, NP_112174). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (100%), Horse, Turkey, Zebra finch, Chicken, Lizard, Xenopus (92%), Opossum (91%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TRIM8

Target: TRIM8 (Tripartite Motif Containing 8 (TRIM8))

Alternative Name: TRIM8 / GERP (TRIM8 Products)

Synonyms: GERP antibody, RNF27 antibody, AA408830 antibody, BB006755 antibody, Rnf27 antibody, si:ch211-208d15.5 antibody, wu:fc90g07 antibody, tripartite motif containing 8 antibody, tripartite motif containing 8 L homeolog antibody, tripartite motif-containing 8 antibody, tripartite motif containing 8b antibody, TRIM8 antibody, trim8.L antibody, Trim8 antibody, trim8b antibody

Background: Name/Gene ID: TRIM8
Family: Tripartite Motif

Synonyms: TRIM8, RING finger protein 27, Tripartite motif-containing 8, Tripartite motif containing 8, GERP, RNF27, Tripartite motif protein TRIM8

Gene ID: 81603

NCBI Accession: NP_112174

UniProt: Q9BZR9