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POLDIP3 antibody (AA 326-375)

POLDIP3 Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Monkey, Bat, Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6741591
  • Target See all POLDIP3 Antibodies
    POLDIP3 (Polymerase (DNA-Directed), delta Interacting Protein 3 (POLDIP3))
    Binding Specificity
    • 15
    • 14
    • 8
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 326-375
    Reactivity
    Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Monkey, Bat, Pig
    Host
    • 57
    Rabbit
    Clonality
    • 55
    • 1
    Polyclonal
    Conjugate
    • 22
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This POLDIP3 antibody is un-conjugated
    Application
    • 45
    • 26
    • 13
    • 13
    • 7
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human POLDIP3 / SKAR
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Chicken (92%) Xenopus (85%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa326-375 of human POLDIP3 (Q9BY77, NP_115687). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Zebra finch, Chicken, Platypus (92%), Xenopus (85%), Sea squirt (84%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product POLDIP3 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (10 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled Water.
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    POLDIP3 (Polymerase (DNA-Directed), delta Interacting Protein 3 (POLDIP3))
    Alternative Name
    POLDIP3 / p46 (POLDIP3 Products)
    Synonyms
    PDIP46 antibody, SKAR antibody, 1110008P04Rik antibody, AA408269 antibody, AL022852 antibody, C77954 antibody, mKIAA1649 antibody, polymerase (DNA-directed), delta interacting protein 3 S homeolog antibody, DNA polymerase delta interacting protein 3 antibody, polymerase (DNA-directed), delta interacting protein 3 antibody, poldip3.S antibody, POLDIP3 antibody, Poldip3 antibody
    Background
    Name/Gene ID: POLDIP3

    Synonyms: POLDIP3, p46, KIAA1649, SKAR, RNA-binding protein P46, PDIP46, S6K1 Aly/REF-like target
    Gene ID
    84271
    NCBI Accession
    NP_115687
    UniProt
    Q9BY77
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