MYL2 antibody (AA 1-166)

Catalog No. ABIN462270

Product Details anti-MYL2 Antibody

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Binding Specificity: AA 1-166

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human MYL2 (1-166aa)

Purification: Protein G purified

Immunogen: Recombinant human MYL2 (1-166aa) purified from E. coli

Type of Immunogen: Recombinant protein

Clone: AT3B2

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Extracts of Rat heart (60 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Target Details for MYL2

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Alternative Name: MYL2 (MYL2 Products)

Synonyms: CMH10 antibody, MLC2 antibody, 2410014J02Rik antibody, MLC-2 antibody, Mlc2 antibody, MLC-2v antibody, Mlc2v antibody, Mylpc antibody, Mlc-2 antibody, myl2 antibody, zgc:136848 antibody, XMLC2 antibody, cmh10 antibody, mlc2 antibody, G2 antibody, MLC-2A antibody, MLC2V antibody, myosin light chain 2 antibody, myosin light chain, phosphorylatable, fast skeletal muscle antibody, myosin, light polypeptide 2, regulatory, cardiac, slow antibody, myosin, light chain 2b, regulatory, cardiac, slow antibody, myosin light chain 2 S homeolog antibody, myosin, light chain 2, regulatory, cardiac, slow antibody, MYL2 antibody, Mylpf antibody, Myl2 antibody, myl2b antibody, myl2.S antibody

Background: Name/Gene ID: MYL2

Synonyms: MYL2, MLC2, Myosin light chain 2, MLC-2, RLC of myosin, CMH10, MLC-2v

Gene ID: 4633

UniProt: P10916

Pathways: Myometrial Relaxation and Contraction