ZNF777 antibody (AA 468-517)

Catalog No. ABIN6741624

Product Details anti-ZNF777 Antibody

Target: ZNF777 (Zinc Finger Protein 777 (ZNF777))

Binding Specificity: AA 468-517

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF777 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ZNF777

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa468-517 of human ZNF777 (Q9ULD5, NP_056509). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Gorilla, Marmoset, Dog, Bat (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ZNF777

Target: ZNF777 (Zinc Finger Protein 777 (ZNF777))

Alternative Name: ZNF777 (ZNF777 Products)

Synonyms: 2500002G23Rik antibody, ZNF777 antibody, RGD1566056 antibody, Znf777 antibody, zinc finger protein 777 antibody, Zfp777 antibody, ZNF777 antibody, LOC769049 antibody

Background: Name/Gene ID: ZNF777
Family: Zinc Finger

Synonyms: ZNF777, Zinc finger protein 777, KIAA1285

Gene ID: 27153

NCBI Accession: NP_056509

UniProt: Q9ULD5