Lysine (K)-Specific Methyltransferase 2B (KMT2B) (AA 2703-2715) antibody

Catalog No. ABIN462281

Product Details

Target: Lysine (K)-Specific Methyltransferase 2B (KMT2B)

Binding Specificity: AA 2703-2715

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Monkey, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human MLL4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Pig, Zebrafish (100%) Xenopus, Pufferfish (92%) Chimpanzee, Elephant, Panda, Dog, Bat, Horse, Opossum, Turkey, Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CNCGAKRCRRFLN from the C-terminus of human MLL4 (NP_055542.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Pig, Zebrafish (100%), Xenopus, Pufferfish (92%), Chimpanzee, Elephant, Panda, Dog, Bat, Horse, Opossum, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: Lysine (K)-Specific Methyltransferase 2B (KMT2B)

Alternative Name: KMT2B / MLL4 (KMT2B Products)

Synonyms: HRX2 antibody, MLL1B antibody, MLL2 antibody, MLL4 antibody, TRX2 antibody, WBP7 antibody, lysine methyltransferase 2B antibody, KMT2B antibody

Background: Name/Gene ID: KMT2B

Synonyms: KMT2B, Lysine N-methyltransferase 2D, MLL4, HRX2, KMT2D, WBP-7, WW domain binding protein 7, Trithorax homologue 2, WBP7, KIAA0304, Lysine N-methyltransferase 2B, Trithorax homolog 2, TRX2, WW domain-binding protein 7

Gene ID: 9757

NCBI Accession: NP_055542

UniProt: Q9UMN6

Pathways: Warburg Effect