FEZ1 antibody (AA 278-290)

Catalog No. ABIN462288

Product Details anti-FEZ1 Antibody

Target: FEZ1 (Fasciculation and Elongation Protein zeta 1 (Zygin I) (FEZ1))

Binding Specificity: AA 278-290

Reactivity: Human, Rat, Dog, Cow, Hamster, Horse, Monkey, Rabbit, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This FEZ1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FEZ1. This antibody is expected to recognize isoform 1 (NP_005094.1) only.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus (100%) Mouse, Pig (92%) Xenopus, Stickleback, Pufferfish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QNKQKEQRELMKK from an internal region of human FEZ1 (NP_005094.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus (100%), Mouse, Pig (92%), Xenopus, Stickleback, Pufferfish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000), WB (1:128000, 0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FEZ1

Target: FEZ1 (Fasciculation and Elongation Protein zeta 1 (Zygin I) (FEZ1))

Alternative Name: FEZ1 (FEZ1 Products)

Synonyms: UNC-76 antibody, UNC76 antibody, wu:fj14c12 antibody, zgc:73258 antibody, fez1 antibody, xfez1 antibody, fasciculation and elongation protein zeta 1 antibody, fasciculation and elongation protein zeta 1 (zygin I) antibody, fasciculation and elongation protein zeta 1 L homeolog antibody, FEZ1 antibody, Fez1 antibody, fez1 antibody, fez1.L antibody

Background: Name/Gene ID: FEZ1

Synonyms: FEZ1, Zygin-1, Zygin I

Gene ID: 9638

NCBI Accession: NP_005094

UniProt: Q99689