GABRA4 antibody (AA 349-361)

Catalog No. ABIN462297

Product Details anti-GABRA4 Antibody

Target: GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))

Binding Specificity: AA 349-361

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GABRA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GABRA4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Elephant, Bovine (92%) Rabbit, Opossum (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EKAKRKTSKPPQE from an internal region of human GABRA4 (NP_000800.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Elephant, Bovine (92%), Rabbit, Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 70kD band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 61.6kD according to NP_000800.2). Recommended concentration: 0.1-0.3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GABRA4

Target: GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))

Alternative Name: GABRA4 (GABRA4 Products)

Synonyms: GABRA4 antibody, Gabra-4 antibody, gamma-aminobutyric acid type A receptor alpha4 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4 antibody, GABRA4 antibody, Gabra4 antibody

Background: Name/Gene ID: GABRA4
Subfamily: GABA A receptor
Family: Ion Channel

Synonyms: GABRA4, GABA A receptor alpha 4, GABA(A) receptor, alpha 4

Gene ID: 2557

NCBI Accession: NP_000800

UniProt: P48169

Pathways: Synaptic Membrane