PPP4C antibody (AA 290-301)
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- Target See all PPP4C Antibodies
- PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))
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Binding Specificity
- AA 290-301
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Reactivity
- Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Zebrafish (Danio rerio), Bat, Hamster, Monkey, Xenopus laevis
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This PPP4C antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human PPP4C.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus, Xenopus, Catfish, Stickleback, Pufferfish, Zebrafish, Water flea (100%) Drosophila, Mosquito, Ant, Beetle (92%) Seq squirt, Poplar, Trichoplax, Toxoplasma, Tick, Spikemoss, Amoeba, Neospora (83%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-PQETRGIPSKKP from the C-terminus of human PPP4C (NP_002711.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus, Xenopus, Catfish, Stickleback, Pufferfish, Zebrafish, Water flea (100%), Drosophila, Mosquito, Ant, Beetle (92%), Seq squirt, Poplar, Trichoplax, Toxoplasma, Tick, Spikemoss, Amoeba, Neospora (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product PPP4C Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA, WB (0.1 - 0.3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot: Approximately 35kD band observed in HEK293, A431 and HeLa lysates (calculated MW of 35.1kD according to NP_002711.1). - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))
- Alternative Name
- PPP4C (PPP4C Products)
- Synonyms
- pp4 antibody, ppx antibody, pp4c antibody, pph3 antibody, MGC75928 antibody, PP4C-A antibody, gc:56413 antibody, ppp4c antibody, wu:fd05e08 antibody, zgc:56413 antibody, PP4 antibody, PP4C antibody, PPH3 antibody, PPP4 antibody, PPX antibody, 1110002D08Rik antibody, AU016079 antibody, Ppx antibody, protein phosphatase 4 catalytic subunit antibody, protein phosphatase 4, catalytic subunit a antibody, protein phosphatase 4, catalytic subunit antibody, protein phosphatase 4 catalytic subunit S homeolog antibody, ppp4c antibody, ppp4ca antibody, PPP4C antibody, Ppp4c antibody, ppp4c.S antibody
- Background
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Name/Gene ID: PPP4C
Subfamily: Protein Phosphatase - Serine/Threonine other
Family: Protein Phosphatase
Synonyms: PPP4C, PP4, PPH3, Protein phosphatase X, PP-X, Protein phosphatase 4, PP4C, PPP4, PPX - Gene ID
- 5531
- NCBI Accession
- NP_002711
- UniProt
- P60510
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