ROR1 antibody

Catalog No. ABIN462403

Product Details anti-ROR1 Antibody

Target: ROR1 (Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ROR1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ROR1. This antibody is expected to recognize isoform 1 (NP_005003.2) only.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Lizard (93%) Turkey, Chicken (87%) Platypus (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-GNKSQKPYKIDSKQA from the C-terminus (near) of human ROR1 (NP_005003.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Lizard (93%), Turkey, Chicken (87%), Platypus (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3 - 6 μg/mL), Peptide-ELISA

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3-6 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ROR1

Target: ROR1 (Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1))

Alternative Name: ROR1 (ROR1 Products)

Synonyms: NTRKR1 antibody, dJ537F10.1 antibody, 2810404D04Rik antibody, Ntrkr1 antibody, mRor1 antibody, RGD1559469 antibody, ROR1 antibody, fi34e02 antibody, si:dkey-209n16.1 antibody, wu:fi34e02 antibody, receptor tyrosine kinase like orphan receptor 1 antibody, receptor tyrosine kinase-like orphan receptor 1 antibody, ROR1 antibody, Ror1 antibody, ror1 antibody

Background: Name/Gene ID: ROR1
Subfamily: Trk/Ror
Family: Protein Kinase

Synonyms: ROR1, DJ537F10.1, NTRKR1, Protein tyrosine kinase t-ror1

Gene ID: 4919

NCBI Accession: NP_005003

UniProt: Q01973

Pathways: RTK Signaling, WNT Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha