TYROBP antibody

Catalog No. ABIN462404

Product Details anti-TYROBP Antibody

Target: TYROBP (TYRO Protein tyrosine Kinase Binding Protein (TYROBP))

Reactivity: Human, Horse, Monkey, Bat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TYROBP antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TYROBP / DAP12. This antibody is expected to recognize both reported isoforms (NP_003323.1, NP_937758.1)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bat, Horse (100%) Sheep, Panda, Rabbit (92%) Mouse, Elephant, Zebu, Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KQRITETESPYQE from an internal region of human TYROBP / DAP12 (NP_003323.1, NP_937758.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bat, Horse (100%), Sheep, Panda, Rabbit (92%), Mouse, Elephant, Zebu, Bovine (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3 - 6 μg/mL), Peptide-ELISA

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3-6 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TYROBP

Target: TYROBP (TYRO Protein tyrosine Kinase Binding Protein (TYROBP))

Alternative Name: DAP12 (TYROBP Products)

Synonyms: TYROBP antibody, DAP12 antibody, tyrpbp antibody, Karap antibody, KARAP antibody, PLOSL antibody, Ly83 antibody, dap12 antibody, TYRO protein tyrosine kinase binding protein antibody, Tyro protein tyrosine kinase binding protein antibody, TYROBP antibody, tyrobp antibody, Tyrobp antibody

Background: Name/Gene ID: TYROBP

Synonyms: TYROBP, DNAX-activation protein 12, DAP12, PLOSL, KARAP, KAR-associated protein, PLO-SL

Gene ID: 7305

NCBI Accession: NP_003323, NP_937758

UniProt: O43914