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HLA-E antibody

HLA-E Reactivity: Human, Primate FACS, IP, IHC, IHC (p) Host: Mouse Monoclonal MEM-E-06 unconjugated
Catalog No. ABIN462415
  • Target See all HLA-E Antibodies
    HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))
    Reactivity
    • 66
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Primate
    Host
    • 44
    • 23
    Mouse
    Clonality
    • 46
    • 21
    Monoclonal
    Conjugate
    • 30
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This HLA-E antibody is un-conjugated
    Application
    • 42
    • 26
    • 19
    • 13
    • 13
    • 11
    • 8
    • 6
    • 3
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Recognized native surface-expressed HLA-E, but not denatured heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7
    Purification
    Protein A purified
    Immunogen
    Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

    Type of Immunogen: Synthetic peptide
    Clone
    MEM-E-06
    Isotype
    IgG1
    Top Product
    Discover our top product HLA-E Primary Antibody
  • Application Notes
    Approved: Flo, IHC, IHC-P (10 μg/mL), IP

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody,
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Tris buffered saline (TBS), 15 mM sodium azide, approx,  pH 8.0. Sourced in Ascites.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    avoid freeze thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • Target
    HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))
    Alternative Name
    HLA-E (HLA-E Products)
    Synonyms
    EA1.2 antibody, EA2.1 antibody, HLA-6.2 antibody, MHC antibody, QA1 antibody, major histocompatibility complex, class I, E antibody, HLA-E antibody, PATR-E antibody
    UniProt
    P13747
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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