eIF4EBP2 antibody (AA 99-120)

Catalog No. ABIN462435

Product Details anti-eIF4EBP2 Antibody

Target: eIF4EBP2 (EIF4EBP2) (Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2))

Binding Specificity: AA 99-120

Reactivity: Human, Mouse, Cow, Dog, Pig, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eIF4EBP2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Detects 17 and 20 kD proteins, corresponding to the apparent molecular mass of PHAS-II and its phosphorylated state on SDS-PAGE immunoblots.

Purification: Immunoaffinity purified

Immunogen: Human PHAS-II synthetic peptide (residues 99-120) was synthesized and the peptide coupled to KLH. This sequence is 96% homologous to mouse PHAS-11 over these residues.

Type of Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: BBS,  pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)

Handling Advice: avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Target Details for eIF4EBP2

Target: eIF4EBP2 (EIF4EBP2) (Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2))

Alternative Name: 4E-BP2 / EIF4EBP2 (EIF4EBP2 Products)

Synonyms: 4ebp2 antibody, MGC75579 antibody, wu:fa55e11 antibody, wu:fp45e09 antibody, MGC78987 antibody, EIF4EBP2 antibody, eIF-4E antibody, Tb06.28P18.1010 antibody, 4EBP2 antibody, PHASII antibody, 2810011I19Rik antibody, 4E-BP2 antibody, AA792569 antibody, BC010348 antibody, PHAS-II antibody, eukaryotic translation initiation factor 4E binding protein 2 antibody, eukaryotic translation initiation factor 4E binding protein 2 L homeolog antibody, eukaryotic initiation factor 4E antibody, eukaryotic translation initiation factor 4E antibody, putative eukaryotic translation initiation factor 4e antibody, eif4ebp2 antibody, Eif4ebp2 antibody, eif4ebp2.L antibody, EIF4EBP2 antibody, eIF-4E antibody, Tb927.6.1870 antibody, LMJF_30_0450 antibody

Background: Name/Gene ID: EIF4EBP2

Synonyms: EIF4EBP2, 4E-BP2, 4EBP2, EIF4E-binding protein 2, PHASII

Gene ID: 1979

UniProt: Q13542