NCKAP1L antibody

Catalog No. ABIN462501

Product Details anti-NCKAP1L Antibody

Target: NCKAP1L (NCK-Associated Protein 1-Like (NCKAP1L))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Monkey, Bat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This NCKAP1L antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Specificity: Human NCKAP1L.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%) Elephant (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human NCKAP1L. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%), Elephant (92%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NCKAP1L

Target: NCKAP1L (NCK-Associated Protein 1-Like (NCKAP1L))

Alternative Name: NCKAP1L (NCKAP1L Products)

Synonyms: HEM1 antibody, 4930568P13Rik antibody, AI463083 antibody, Hem1 antibody, Hemp1 antibody, NCK associated protein 1 like antibody, zgc:172352 antibody, nck-associated protein 1-like antibody, NCK-associated protein 1-like antibody, NCKAP1L antibody, zgc:172352 antibody, LOC100030968 antibody, nckap1l antibody, LOC100618382 antibody, LOC100639088 antibody, Nckap1l antibody

Background: Name/Gene ID: NCKAP1L

Synonyms: NCKAP1L, HEM1, Hematopoietic protein 1, NCK-associated protein 1-like

Gene ID: 3071

UniProt: P55160

Pathways: Regulation of Actin Filament Polymerization