PDHB antibody (AA 72-121)

Catalog No. ABIN6741021

Product Details anti-PDHB Antibody

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Binding Specificity: AA 72-121

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Bat, Chicken, Monkey, Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDHB antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human PDHB

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Horse, Pig, Chicken (100%) Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human PDHB (P11177, NP_000916). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Lizard, Catfish (100%), Zebra finch, Salmon, Smelt, Stickleback, Pike, Pufferfish, Zebrafish, Drosophila (92%), Xenopus, Beetle (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PDHB

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Alternative Name: PDHB (PDHB Products)

Synonyms: CG 11876 antibody, Dmel\\CG11876 antibody, E1beta antibody, PDHB antibody, zgc:64062 antibody, wu:fc76a05 antibody, PDHBD antibody, PDHE1-B antibody, PHE1B antibody, 2610103L06Rik antibody, AL024199 antibody, C81408 antibody, CG11876 gene product from transcript CG11876-RA antibody, pyruvate dehydrogenase E1 beta subunit antibody, pyruvate dehydrogenase (lipoamide) beta antibody, CG11876 antibody, pdhb antibody, PDHB antibody, Pdhb antibody

Background: Name/Gene ID: PDHB

Synonyms: PDHB, PDHE1-B, PDHBD, PHE1B

Gene ID: 5162

NCBI Accession: NP_000916

UniProt: P11177

Pathways: Warburg Effect