Septin 12 antibody (AA 180-229)

Catalog No. ABIN6741160

Product Details anti-Septin 12 Antibody

Target: Septin 12 (Sep12)

Binding Specificity: AA 180-229

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Bat, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Septin 12 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human SEPT12 / Septin 12

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Dog, Bovine (92%) Salmon, Pufferfish, Zebrafish (90%) Xenopus (84%) Neurospora, Trichoplax, Tunicate, Paracoccidioides (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human SEPT12 (Q8IYM1, NP_653206). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Gibbon, Dog, Opossum (92%), Platypus (91%), Turkey, Salmon, Stickleback, Zebrafish (90%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Septin 12

Target: Septin 12 (Sep12)

Alternative Name: SEPT12 / Septin 12 (Sep12 Products)

Synonyms: MGC68931 antibody, fe23c11 antibody, si:dkey-23a11.2 antibody, wu:fe23c11 antibody, SPGF10 antibody, 1700028G04Rik antibody, 4933413B09Rik antibody, Septin12 antibody, septin 12 S homeolog antibody, septin 12 antibody, sept12.S antibody, SEPT12 antibody, sept12 antibody, Sept12 antibody

Background: Name/Gene ID: SEPT12

Synonyms: SEPT12, Septin-12, Septin 12, SPGF10

Gene ID: 124404

NCBI Accession: NP_653206

UniProt: Q8IYM1