EN1 antibody (AA 252-301)

Catalog No. ABIN6741515

Product Details anti-EN1 Antibody

Target: EN1 (Engrailed Homeobox 1 (EN1))

Binding Specificity: AA 252-301

Reactivity: Human, Mouse, Chicken, Rat, Guinea Pig, Cow, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EN1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human EN1

Predicted Reactivity: Percent identity by BLAST analysis: Pig, Guinea pig (93%) Xenopus (92%) Rabbit (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa252-301 of human EN1 (Q05925, NP_001417). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Bovine, Pig, Guinea pig, Chicken, Platypus, Lizard (100%), Gibbon, Galago, Elephant, Bat, Horse, Opossum (93%), Rabbit, Xenopus, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EN1

Target: EN1 (Engrailed Homeobox 1 (EN1))

Alternative Name: EN1 / Engrailed (EN1 Products)

Synonyms: EN1 antibody, En-1 antibody, Mo-en.1 antibody, ENG-1 antibody, en-1 antibody, en1 antibody, eng1 antibody, zgc:100771 antibody, en1-A antibody, engrailed-1 antibody, xen1 antibody, Gg-En-1 antibody, en-3 antibody, engrailed antibody, engrailed homeobox 1 antibody, engrailed 1 antibody, engrailed homeobox 1a antibody, engrailed homeobox 1 S homeolog antibody, EN1 antibody, En1 antibody, en1a antibody, en1.S antibody

Background: Name/Gene ID: EN1

Synonyms: EN1, Engrailed 1, Engrailed homolog 1, Homeobox protein en-1, Homeobox protein engrailed-1, Hu-En-1, Engrailed homeobox 1

Gene ID: 2019

NCBI Accession: NP_001417

UniProt: Q05925

Pathways: Dopaminergic Neurogenesis