FGF10 antibody

Catalog No. ABIN464942

Product Details anti-FGF10 Antibody

Target: FGF10 (Fibroblast Growth Factor 10 (FGF10))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FGF10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human FGF-10

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human FGF-10.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1 μg/mL-2.5 μg/mL with an overnight iincubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hFGF-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-10 (LS-C104659) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-10. Western Blot: To detect hFGF-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for FGF10

Target: FGF10 (Fibroblast Growth Factor 10 (FGF10))

Alternative Name: FGF10 (FGF10 Products)

Synonyms: fgf10 antibody, fgf-10 antibody, wu:fd11d03 antibody, zgc:109774 antibody, FGF10 antibody, AEY17 antibody, BB213776 antibody, Fgf-10 antibody, Gsfaey17 antibody, FGF-10 antibody, fibroblast growth factor 10a antibody, fibroblast growth factor 10 antibody, fgf10a antibody, FGF10 antibody, fgf10 antibody, Fgf10 antibody

Background: Name/Gene ID: FGF10
Family: HBGF

Synonyms: FGF10, Fibroblast growth factor 10, FGF-10, Keratinocyte growth factor 2

Gene ID: 2255

UniProt: O15520

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Tube Formation, Positive Regulation of Response to DNA Damage Stimulus