PLGF antibody (Biotin)

Catalog No. ABIN465295

This Rabbit Polyclonal antibody specifically detects PLGF in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PLGF antibody (Biotin) (ABIN465295)

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLGF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-PLGF Antibody (Biotin)

Specificity: Human PlGF-1

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human PlGF-1.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hPlGF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPlGF. To detect hPlGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human PlGF as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPlGF. Western Blot: To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPlGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for PLGF

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Alternative Name: PGF / PLGF

Background: Name/Gene ID: PGF
Family: PDGF

Synonyms: PGF, D12S1900, PGFL, PlGF-2, Placental growth factor, Placental growth factor-like, PLGF, Placenta growth factor, SHGC-10760

Gene ID: 5228

UniProt: P49763

Pathways: VEGFR1 Specific Signals