Cardiotrophin 1 antibody

Catalog No. ABIN465487

Product Details anti-Cardiotrophin 1 Antibody

Target: Cardiotrophin 1 (CTF1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cardiotrophin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Human Cardiotrophin-1

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Cardiotrophin-1.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, Neut (0.25 - 0.4 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hCardiotrophin-1 (15 ng/mL), a concentration of 0.25-0.40 μg/mL of this antibody is required. ELISA: To detect hCT-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CT-1 (LS-C104694) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCT-1. Western Blot: To detect hCT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCT-1 is 1.5-3 ng/lane, under reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Cardiotrophin 1

Target: Cardiotrophin 1 (CTF1)

Alternative Name: CFT1 / CT-1 / Cardiotrophin-1 (CTF1 Products)

Synonyms: CTF1 antibody, ct1 antibody, ct-1 antibody, ctf2 antibody, CT-1 antibody, CT1 antibody, cardiotrophin 1 antibody, cardiotrophin-1 antibody, CTF1 antibody, ctf1 antibody, Ctf1 antibody, LOC100736818 antibody

Background: Name/Gene ID: CTF1

Synonyms: CTF1, Cardiotrophin 1, Cardiotrophin-1, CT1, Cardiophin 1, CT-1

Gene ID: 1489

UniProt: Q16619

Pathways: JAK-STAT Signaling