Neurotrophin 4 antibody (Biotin)

Catalog No. ABIN465543

Product Details anti-Neurotrophin 4 Antibody (Biotin)

Target: Neurotrophin 4 (NTF4)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Neurotrophin 4 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Human NT-4

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human NT-4.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hNT-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hNT-4. To detect hNT-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human NT-4 (LS-C104651) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNT-4. Western Blot: To detect hNT-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Neurotrophin 4

Target: Neurotrophin 4 (NTF4)

Alternative Name: NTF4 / NT4 / Neurotrophin 4 (NTF4 Products)

Synonyms: NTF5 antibody, GLC10 antibody, GLC1O antibody, NT-4 antibody, NT-4/5 antibody, NT-5 antibody, NT4 antibody, NT5 antibody, NT4P antibody, Ntf5 antibody, 2900040K06Rik antibody, AI462899 antibody, NT4/5 antibody, Ntf-5 antibody, Ntf4 antibody, neurotrophin 4 antibody, neurotrophin 5 antibody, NTF4 antibody, Ntf4 antibody, ntf4 antibody, Ntf5 antibody

Background: Name/Gene ID: NTF4
Family: NGF Beta

Synonyms: NTF4, GLC1O, GLC10, Neurotrophic factor 5, NT-4, Neurotrophic factor 4, Neurotrophin-4, Neurotrophin-4 precursor, NT-5, NT4, NT5, NTF5, Neurotrophin 4, Neurotrophin-5, Neutrophic factor 4, NT-4/5

Gene ID: 4909

UniProt: P34130

Pathways: RTK Signaling