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PDGF-BB Homodimer antibody

Reactivity: Human WB, ELISA, Neut Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN465589
  • Target
    PDGF-BB Homodimer
    Reactivity
    • 39
    • 25
    • 21
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 42
    • 4
    • 1
    Rabbit
    Clonality
    • 43
    • 4
    Polyclonal
    Conjugate
    • 20
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Un-conjugated
    Application
    • 47
    • 17
    • 13
    • 13
    • 11
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Neutralization (Neut)
    Specificity
    Human PDGF-BB
    Purification
    Immunoaffinity purified
    Immunogen
    E.coli derived recombinant Human PDGF-BB.

    Type of Immunogen: Cell extract
  • Application Notes
    Approved: ELISA, Neut (3 - 4 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPDGF-BB (2.5 ng/mL), a concentration of 0.03-0.04 μg/mL of this antibody is required. ELISA: To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PDGF-BB (LS-C104638) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Sterile water to a concentration of 0.1-1.0 mg/mL
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS,  pH 7.2
    Handling Advice
    Avoid freeze/thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • Target
    PDGF-BB Homodimer
    Alternative Name
    PDGF-BB
    Synonyms
    PDGF-2 antibody, PDGF2 antibody, SIS antibody, SSV antibody, c-sis antibody, PDGF-B antibody, Sis antibody, platelet derived growth factor subunit B antibody, platelet derived growth factor, B polypeptide antibody, PDGFB antibody, Pdgfb antibody
    Background
    Name/Gene ID: PDGFB
    Family: PDGF

    Synonyms: PDGFB, Becaplermin, PDGF-BB, PDGF-b, SSV, PDGF subunit B, PDGF-2, PDGF2, Proto-oncogene c-Sis, SIS, C-sis, PDGF, B chain
    Gene ID
    5155
    UniProt
    P01127
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