CNTF antibody

Catalog No. ABIN465750

Product Details anti-CNTF Antibody

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNTF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Rat CNTF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Rat CNTF.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (2 - 5 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (including the globus pallidus region) tissue. The primary antibody was incubated at 0.25 mg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat CNTF (0.15 ng/mL), a concentration of 0.02-0.05 μg/mL of this antibody is required. ELISA: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat CNTF (LS-C104736) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western Blot: To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CNTF

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Alternative Name: CNTF (CNTF Products)

Synonyms: GPA antibody, CNTF antibody, HCNTF antibody, AI429687 antibody, ciliary neurotrophic factor antibody, CNTF antibody, Cntf antibody

Background: Name/Gene ID: CNTF

Synonyms: CNTF, Ciliary neurotrophic factor, HCNTF

Gene ID: 1270

UniProt: P26441

Pathways: JAK-STAT Signaling