KIT Ligand antibody (Biotin)

Catalog No. ABIN465802

Product Details anti-KIT Ligand Antibody (Biotin)

Target: KIT Ligand (KITLG)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT Ligand antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Mouse SCF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine SCF.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mSCF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mSCF. To detect mSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine SCF (LS-C104783) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mSCF. Western Blot: To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: KITLG / SCF (KITLG Products)

Background: Name/Gene ID: KITLG

Synonyms: KITLG, C-Kit ligand, KIT ligand, Kitl, KL-1, SHEP7, SF, Steel factor, Stem cell factor, SCF, FPH2, Mast cell growth factor, MGF

Gene ID: 4254

UniProt: P21583

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway