Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
Human DPYSL3 / CRMP4
Predicted Reactivity
Percent identity with other species by BLAST analysis: Human (100%), Monkey (100%), Mouse (100%), Rat (100%), Dog (100%), Bovine (100%), Chicken (85%).
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide from internal region of human DPYSL3 (NP_001378.1, Q14195). Percent identity with other species by BLAST analysis: Human (100%), Monkey (100%), Mouse (100%), Rat (100%), Dog (100%), Bovine (100%), Chicken (85%).
DPYSL3
Reactivity: Human
ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Approved: IHC-P (10 μg/mL), WB (1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Distilled water
Concentration
Lot specific
Buffer
Lyophilized from PBS with 2 % sucrose
Handling Advice
Avoid repeat freeze-thaw cycles.
Storage
4 °C,-20 °C
Storage Comment
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year) Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.