Pleckstrin antibody (N-Term)

Catalog No. ABIN6741695

Product Details anti-Pleckstrin Antibody

Target: Pleckstrin (PLEK)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Guinea Pig, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pleckstrin antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human PLEK

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig, Zebrafish (100%) Dog, Horse, Chicken (92%) Xenopus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human PLEK (P08567, NP_002655). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Bovine, Bat, Pig, Guinea pig, Lizard, Zebrafish (100%), Monkey, Elephant, Dog, Horse, Opossum, Turkey, Zebra finch, Chicken, Platypus (92%), Xenopus, Trout, Salmon, Stickleback (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Pleckstrin

Target: Pleckstrin (PLEK)

Alternative Name: PLEK / Pleckstrin (PLEK Products)

Background: Name/Gene ID: PLEK

Synonyms: PLEK, Platelet 47 kDa protein, Pleckstrin, p47

Gene ID: 5341

NCBI Accession: NP_002655

UniProt: P08567

Pathways: Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process