VPS53 antibody (AA 431-480)

Catalog No. ABIN6742156

Product Details anti-VPS53 Antibody

Target: VPS53 (Vacuolar Protein Sorting 53 Homolog (VPS53))

Binding Specificity: AA 431-480

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Zebrafish (Danio rerio), Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS53 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human VPS53

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse (100%) Dog, Guinea pig, Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa431-480 of human VPS53 (B3FH42, NP_001121631). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Mouse, Rat, Hamster, Panda, Bovine, Rabbit, Horse, Opossum, Platypus (100%), Elephant, Dog, Bat, Guinea pig, Zebra finch, Chicken (92%), Pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for VPS53

Target: VPS53 (Vacuolar Protein Sorting 53 Homolog (VPS53))

Alternative Name: HCCS1 / VPS53 (VPS53 Products)

Synonyms: wu:fb77d11 antibody, zgc:103741 antibody, DKFZp469N152 antibody, 2010002A08Rik antibody, 2310040I21Rik antibody, 3100002B05Rik antibody, Hccs1 antibody, HCCS1 antibody, hVps53L antibody, pp13624 antibody, RGD1311391 antibody, VPS53, GARP complex subunit antibody, vacuolar protein sorting 53 homolog (S. cerevisiae) antibody, vacuolar protein sorting 53 antibody, VPS53 GARP complex subunit antibody, VPS53 antibody, vps53 antibody, PAAG_00802 antibody, MCYG_06020 antibody, VDBG_07188 antibody, MGYG_05943 antibody, Vps53 antibody

Background: Name/Gene ID: VPS53

Synonyms: VPS53, HCCS1, HVps53L, Pp13624

Gene ID: 55275

NCBI Accession: NP_001121631

UniProt: Q5VIR6