FZD1 antibody (Extracellular Domain) (Biotin)
-
- Target See all FZD1 (Fzd1) Antibodies
- FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))
-
Binding Specificity
- Extracellular Domain
-
Reactivity
- Human, Mouse
-
Host
- Rat
-
Clonality
- Monoclonal
-
Conjugate
- This FZD1 antibody is conjugated to Biotin
-
Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Specificity
- Recognizes mouse Frizzled-1 extracellular domain. Shows no cross-reactivity with rmFrizzled-2, -3, -4, -6, -7 and -8. Detected Frizzled-1 on differentiated embryoid bodies derived from mouse embryonic cell line D3 by Flow Cytometry. Species cross-reactivity: Human.
- Purification
- Protein G purified
- Immunogen
- NS0-derived rmFrizzled-1 extracellular domain.
- Isotype
- IgG2a
- Top Product
- Discover our top product Fzd1 Primary Antibody
-
-
- Application Notes
-
Approved: Flo, ICC, WB
Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 10 μL of this solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 mL of a 25 mg/mL stock solution of a secondary developing reagent such as streptavidin conjugated to a fluorochrome. Immunocytochemistry: Used with appropriate secondary reagents at 5-10 μg/mL in 4 % . paraformaldehyde-fixed cells. Western Blot: Use at 1-2 μg/mL with appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~5 ng/lane under non-reducing and reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
-
Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, BSA.
- Storage
- 4 °C,-20 °C
- Storage Comment
-
Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
-
- Target
- FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))
- Alternative Name
- FZD1 / Frizzled 1 (Fzd1 Products)
- Synonyms
- CG17697 antibody, CG3646 antibody, DFZ1 antibody, DFz antibody, DFz1 antibody, Dfz1 antibody, Dm Fz1 antibody, Dmel\\CG17697 antibody, FZ antibody, Frz antibody, Fz antibody, Fz1 antibody, frz antibody, fz1 antibody, fz[[1]] antibody, Fz-1 antibody, cFz-1 antibody, Xfrizzled-1 antibody, frizzled1 antibody, frz-1 antibody, frz1 antibody, fzd1-A antibody, AW227548 antibody, FZ-1 antibody, frizzled antibody, frizzled class receptor 1 antibody, frizzled class receptor 1 L homeolog antibody, fz antibody, FZD1 antibody, Fzd1 antibody, fzd1.L antibody
- Background
-
Name/Gene ID: FZD1
Subfamily: Frizzled
Family: GPCR
Synonyms: FZD1, Chfz-1, Fz1, Frizzled 1, Fz receptor 1, HFz1, Frizzled homolog 1, Fz-1, FzE1, Rfz1, Xfrizzled-1, Wnt receptor, Dfrizzled-1, Frizzled family receptor 1, Frizzled-1 - Gene ID
- 8321
- UniProt
- Q9UP38
- Pathways
- WNT Signaling, Asymmetric Protein Localization
-