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Antibodies
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anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma...
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr1221/Tyr1222) antibody
| Antigen |
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| Synonyms |
Neu, HER2, HER-2, c-neu, Erbb-2, c-erbB2, mKIAA3023, NEU, NGL, TKR1, CD340, HER-2/neu |
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Epitope
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pTyr1248 (16), pTyr877 (10), Ab-1221/1222 (5), pTyr1112 (5), C-Term (4), pTyr1221/Tyr1222 (4), pTyr1221/1222 (3), AA 1240-1260 (2), pSer1113 (2), pThr686 (2), AA 1243-1255 (1), AA 153-433 (1), N-Term (1), pTyr1139 (1)
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA
Western Blotting (WB) (142), Immunofluorescence (IF) (71), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (67), Immunohistochemistry (IHC) (58), Enzyme Immunoassay (EIA) (35), Immunoprecipitation (IP) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), ELISA (13), Flow Cytometry (FACS) (13), Functional Studies (Func) (4), Dot Blot (Dot) (1), Radioimmunoassay (RIA) (1)
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| Catalog no. |
ABIN482471 |
| Quantity |
50µg (1 mg/ml) |
| Price |
150.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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HER2 (Phospho-Tyr1221/Tyr1222)
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Gene ID
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2064 (NCBI Gene Symbol: ERBB2)
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Swiss-Prot
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P04626
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Immunogen
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The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1221/Tyr1222.
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Format
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Liquid
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Synonyms: C-erbB-2, Epidermal growth factor receptor-related protein, ErbB2, HER2, kinase ErbB2, MLN 19, NEU, NEU proto-oncogene, NGL, p185erbB2, Receptor protein-tyrosine kinase erbB-2, Receptor protein-tyrosine kinase erbB-2 precursor, Tyrosine kinase-type cell surface receptor HER2, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
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Specificity
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HER2 (Phospho-Tyr1221/Tyr1222) Antibody detects endogenous levels of HER2 only when phosphorylated at tyrosine1221/tyrosine1222.
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Molecular Weight
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137 kDa
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Comments
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OMIM-Number: 164870, Unigene-Number: Hs.446352
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
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Concentration
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1 mg/ml
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Purity
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Greater 95%
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
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Buffer
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Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C/1 year
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Restrictions
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For Research Use only
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Alternatives for antigen "V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2)", type "Antibodies"
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Hosts
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Rabbit (119), Mouse (58), Rat (4), Sheep (3), Chicken (2)
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Reactivities
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Human (172), Rat (Rattus) (72), Mouse (Murine) (69), Monkey (7), Dog (Canine) (6)
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Applications
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Western Blotting (WB) (142), Immunofluorescence (IF) (71), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (67), Immunohistochemistry (IHC) (58), Enzyme Immunoassay (EIA) (35), Immunoprecipitation (IP) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), ELISA (13), Flow Cytometry (FACS) (13), Functional Studies (Func) (4), Dot Blot (Dot) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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Biotin (3), FITC (1)
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Epitopes
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pTyr1248 (16), pTyr877 (10), Ab-1221/1222 (5), pTyr1112 (5), C-Term (4), pTyr1221/Tyr1222 (4), pTyr1221/1222 (3), AA 1240-1260 (2), pSer1113 (2), pThr686 (2), AA 1243-1255 (1), AA 153-433 (1), N-Term (1), pTyr1139 (1)
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