NK2 Homeobox 5 (NKX2-5) (N-Term) antibody

Details for Product No. ABIN486853
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Antigen
Synonyms NKX2-5, Nkx2.5, Nkx2-5, csx, csx1, nkx2e, nkx2.5, tinman, Nkx-2.5, XNkx2-5, XNkx-2.5, CHNG5, CSX, CSX1, HLHS2, NKX2.5, NKX2E, NKX4-1, VSD3, Csx, CNKX-2.5, NKX-2.5
Epitope
N-Term
(9), (8), (7), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Rabbit, Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine), Human
(63), (20), (16), (13), (13), (11), (1), (1)
Host
Rabbit
(36), (25), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(53), (35), (9), (5), (4), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN486853
289.00 $
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Immunogen Synthetic peptide directed towards the n terminal of human Nkx2-5
Sequence ELGRAPSPAK CASAFPAAPA FYPRAYSDPD PAKDPRAEKK ELCALQKAVE
Predicted Reactivity Human : 100 %, Mouse : 90 %, Rat : 90 %, Guinea pig : 90 %, Bovine : 80 %, Dog : 80 %, Rabbit : 70 %
Characteristics This is a rabbit polyclonal antibody against NKX2-5. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name Nkx2-5
Background Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.
Molecular Weight 35 kDa
Gene ID 1482
NCBI Accession NP_004378, NM_004387
UniProt P52952
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 324 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-NK2 Homeobox 5 (NKX2-5) (N-Term) antibody WB Suggested Anti-Nkx2-5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: MCF7 cell lysate
Background publications Nagel, Meyer, Quentmeier et al.: "MEF2C is activated by multiple mechanisms in a subset of T-acute lymphoblastic leukemia cell lines." in: Leukemia, Vol. 22, Issue 3, pp. 600-7, 2008 (PubMed).

Validation Images
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