MDS1 and EVI1 Complex Locus (MECOM) (N-Term) antibody

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Antigen
Synonyms evi1, im:7140716, prdm3, EVI1, AML1-EVI-1, MDS1, MDS1-EVI1, PRDM3, D630039M04Rik, Evi-1, Evi1, Jbo, Mds, Mds1, Mds1-Evi1, Prdm3, Znfpr1b1, AML1, AMLCR1, CBFA2, EVI-1, PEBP2aB
Epitope
N-Term
(36), (27), (17), (16), (10), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(105), (23), (7), (3), (2), (2)
Host
Rabbit
(72), (32), (12)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (6), (6), (6)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(103), (63), (10), (6), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human EVI1
Blocking Peptide Blocking peptide for this product available: MDS1 and EVI1 Complex Locus (MECOM) peptide (ABIN976487)
Sequence THPQILPATQ DILKALSKHP SVGDNKPVEL QPERSSEERP FEKISDQSES
Predicted Reactivity Human : 100 %, Mouse : 80 %
Characteristics This is a rabbit polyclonal antibody against EVI1. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Alternative Name EVI1 (MECOM Antibody Abstract)
Background EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity.
Molecular Weight 118 kDa
Gene ID 2122
NCBI Accession NM_005241, NP_005232
UniProt Q03112
Research Area Chromatin and Nuclear Signaling, Chromatin Binding Proteins, Cell Cycle
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1051 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-MDS1 and EVI1 Complex Locus (MECOM) (N-Term) antibody (ABIN486917) WB Suggested Anti-EVI1 Antibody Titration: 0.2-1 ug/ml
Positive Control:...
Immunohistochemistry (IHC) image for anti-MDS1 and EVI1 Complex Locus (MECOM) (N-Term) antibody (ABIN486917) anti-MDS1 and EVI1 Complex Locus (MECOM) (N-Term) antibody (Image 2)
Background publications Chi, Senyuk, Chakraborty et al.: "EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity." in: The Journal of biological chemistry, Vol. 278, Issue 50, pp. 49806-11, 2003 (PubMed).

Catalog No. ABIN486917
$289.00
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