anti-ZEB1 antibody (Zinc Finger E-Box Binding Homeobox 1) (N-Term)

Details for Product anti-ZEB1 Antibody No. ABIN486932, Supplier: Login to see New
New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
ZFH/[d]EF1, kheper, zeb1, zfhx1, bzp, zeb, tcf8, areb6, nil2a, zfhep, nil-2a, zfhx1a, nil-2-a, MGC97552, TCF8, AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, 3110032K11Rik, MEB1, Nil2, TCF- ... show more
ZFH/[d]EF1, kheper, zeb1, zfhx1, bzp, zeb, tcf8, areb6, nil2a, zfhep, nil-2a, zfhx1a, nil-2-a, MGC97552, TCF8, AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, 3110032K11Rik, MEB1, Nil2, TCF-8, Tcf18, Tcf8, Tw, ZEB, Zfhep, Zfhx1a, Zfx1a, Zfx1ha, [delta]EF1, deltaEF1, deltaef1, fecd6 show less
Epitope
N-Term
(20), (12), (8), (8), (6), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(115), (28), (26), (1), (1), (1), (1), (1)
Host
Rabbit
(56), (44), (15)
Clonality
Polyclonal
Conjugate
This ZEB1 antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(92), (38), (15), (13), (13), (11), (11), (10), (4), (3), (3), (2), (1), (1)
Pubmed 2 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 50 μg
Options
Shipping to United States ( )
Availability Will be delivered in 2 to 3 Business Days
Immunogen Synthetic peptide directed towards the N terminal of human ZEB1
Blocking Peptide Blocking peptide for this product available: Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (N-Term) peptide (ABIN986406)
Sequence KDDECESDAE NEQNHDPNVE EFLQQQDTAV IFPEAPEEDQ RQGTPEASGH
Predicted Reactivity Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, African clawed frog : 90 %, Bovine : 90 %
Characteristics This is a rabbit polyclonal antibody against ZEB1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name ZEB1 (ZEB1 Antibody Abstract)
Background ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).
Molecular Weight 124 kDa
Gene ID 6935
NCBI Accession NP_110378, NM_030751
UniProt P37275
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1124 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-ZEB1 antibody (Zinc Finger E-Box Binding Homeobox 1) (N-Term) (ABIN486932) WB Suggested Anti-ZEB1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Image no. 2 for anti-ZEB1 antibody (Zinc Finger E-Box Binding Homeobox 1) (N-Term) (ABIN486932) anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (N-Term) antibody (Image 2)
Image no. 3 for anti-ZEB1 antibody (Zinc Finger E-Box Binding Homeobox 1) (N-Term) (ABIN486932) anti-Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (N-Term) antibody (Image 3)
Product cited in: Lobert, Graichen, Morris: "Coordinated regulation of β-tubulin isotypes and epithelial-to-mesenchymal transition protein ZEB1 in breast cancer cells." in: Biochemistry, Vol. 52, Issue 32, pp. 5482-90, 2013 (PubMed).

Background publications Gregory, Bert, Paterson et al.: "The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1." in: Nature cell biology, Vol. 10, Issue 5, pp. 593-601, 2008 (PubMed).

Catalog No. ABIN486932
$289.00
Plus shipping costs $45.00

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)