Sirtuin 4 (SIRT4) (Middle Region) antibody

Details for Product No. ABIN486937
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Antigen
Synonyms SIRT4, im:7139453, SIR2L4, 4930596O17Rik
Epitope
Middle Region
(18), (7), (6), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Pig (Porcine), Human
(49), (32), (31), (16), (14), (2)
Host
Rabbit
(37), (9), (8), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(46), (34), (12), (9), (7), (6), (4), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN486937
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Immunogen Synthetic peptide directed towards the middle region of human SIRT4
Sequence QVPTCVQCGG HLKPDVVFFG DTVNPDKVDF VHKRVKEADS LLVVGSSLQV
Predicted Reactivity Human : 100 %, Bovine : 90 %, Mouse : 90 %, Rat : 90 %, Sheep : 90 %, Pig : 80 %, Dog : 70 %
Characteristics This is a rabbit polyclonal antibody against SIRT4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name SIRT4
Background SIRT4 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. SIRT4 is included in class IV of the sirtuin family.This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
Molecular Weight 35 kDa
Gene ID 23409
NCBI Accession NP_036372, NM_012240
UniProt Q9Y6E7
Research Area Chromatin and Nuclear Signaling, Metabolism, Proteases
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 314 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Sirtuin 4 (SIRT4) (Middle Region) antibody WB Suggested Anti-SIRT4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: MCF7 cell lysate
anti-Sirtuin 4 (SIRT4) (Middle Region) antibody (2) anti-Sirtuin 4 (SIRT4) (Middle Region) antibody (Image 2)
anti-Sirtuin 4 (SIRT4) (Middle Region) antibody (3) Host: Rabbit
Target Name: SIRT4
Sample Tissue: MCF7Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1 µg/mL Peptide Concentration: 5 µg/mL Lysate Quantity: 41 µg/lane/laneGel Concentration:.12
Background publications Ahuja, Schwer, Carobbio et al.: "Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase." in: The Journal of biological chemistry, Vol. 282, Issue 46, pp. 33583-92, 2007 (PubMed).

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