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PAP Associated Domain Containing 4 (PAPD4) (N-Term) antibody

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Antigen
Synonyms GLD2, 8030446C20Rik, AI649009, GLD-2, Gld2, zgc:110560
Epitope
N-Term
(19), (15), (3), (2)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(23), (5), (5), (3)
Host
Rabbit
(24)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(22), (15), (14), (2)
Pubmed 2 references available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human PAPD4
Blocking Peptide Blocking peptide for this product available: ABIN981337
Sequence GRKRLSDEKN LPLDGKRQRF HSPHQEPTVV NQIVPLSGER RYSMPPLFHT
Predicted Reactivity Dog : 100 %, Horse : 100 %, Human : 100 %, Pig : 100 %
Characteristics This is a rabbit polyclonal antibody against PAPD4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name PAPD4 (PAPD4 Antibody Abstract)
Background PAPD4 is a cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. PAPD4 belongs to the DNA polymerase type-B-like family, GLD2 subfamily.
Molecular Weight 56 kDa
Gene ID 167153
NCBI Accession NM_173797, NP_776158
UniProt Q6PIY7
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 484 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-PAP Associated Domain Containing 4 (PAPD4) (N-Term) antibody (ABIN487221) WB Suggested Anti-PAPD4 Antibody Titration: 0.2-1 ug/ml
Positive Control...
Product cited in: Lazrek, Goffard, Schanen et al.: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, 2006 (PubMed).

Background publications Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Catalog No. ABIN487221
$289.00
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