CD161 antibody (FITC)

Catalog No. ABIN487477

Product Details anti-CD161 Antibody (FITC)

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD161 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts with Human CD161 antigen.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: HNKR-P1a, CLEC5B, NKRP1A, Killer cell lectin-like receptor subfamily B member 1, Naturalkiller cell surface protein P1A, C-type lectin domain family 5 member B

Purification: Protein-A Sepharose Chromatography.

Immunogen: Human polyclonal NK cells. Remarks: Hybridoma was established by fusion of mouse myeloma cell SP2 with Balb/cmouse splenocyte.

Clone: HP-3G10

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 10-20 μg/mL (final concentration). Positive Control: Peripheral blood Lymphocyte cells. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Flow Cytometric analysis for floating cellsProtocol 1. We use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Add 10 μL of normal goat serum to the cell pellet after tapping. Mix well, and incubatefor 5 minutes at room temperature (20~25°C). 3) Add 30 µL of the FITC labeled CD161 monoclonal antibody (10-20 μg/mL) diluted with thewashing buffer. Mix well, and incubate for 30 minutes at room temperature (20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 μL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum and 0. 1% NaN3(5x10e6 cells/mL). 3) Add 20 µL of the non diluted FITC labeled CD161 monoclonal antibody into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Flow Cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all steps described below. 1) Add 20 μL of the non diluted FITC labeled CD161 monoclonal antibody into each tube. 2) Add 50 μL of whole blood into each tube. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 3) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective packageinserts. 4) Resuspend the cells with 500 μL of the washing buffer [PBS containing 2% fetal calfserum (FCS) and 0. 1% NaN3] and analyze by a flow cytometer. Positive Control for Flow Cytometry: Peripheral blood Lymphocytes

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.05 mg/mL

Buffer: PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD161

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Alternative Name: CD161 / KLRB1 (KLRB1 Products)

Synonyms: NKR-P1 antibody, KLRB1 antibody, 4930431A04Rik antibody, Gm4696 antibody, Klrb1g antibody, Klrb6 antibody, Ly-55 antibody, Ly55 antibody, NKR-P1G antibody, Nkrp-1e antibody, Nkrp1g antibody, CD161 antibody, CLEC5B antibody, NKR antibody, NKR-P1A antibody, NKRP1A antibody, hNKR-P1A antibody, Klrb1d antibody, Klrb1f antibody, Nkr-p1e antibody, killer cell lectin like receptor B1 antibody, killer cell lectin-like receptor subfamily B member 1 antibody, killer cell lectin-like receptor subfamily B, member 1 antibody, KLRB1 antibody, Klrb1 antibody

Background: CD161, also known as NKR-P1, is a receptor originally identified on the surface of natural killer cells that is also present on some subpopulations of CD8+T cells. CD161 is often used as an NK cell marker since it is present on both immature and mature NK cells. CD161 appears to mediate NK cell activation and is up-regulated by IL-12. CD161 is expressed as a disulfide homodimer of ~60 kDa while the ligand for CD161 appears to be a carbohydrate structure that includes GalNAc and GlcNAc structures. CD161+ T cells are commonly found in human liver and intestinal epithelium where they may play a role in local immunity.Synonyms: C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, NKRP1A, Natural killer cell surface protein P1A

Gene ID: 3820

UniProt: Q12918