Rhodopsin antibody (C-Term)

Catalog No. ABIN487512

Product Details anti-Rhodopsin Antibody

Target: Rhodopsin (RHO)

Binding Specificity: C-Term

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Rhodopsin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: Recognizes Rhodopsin (~40? kDa). Reacts with both native and recombinant protein. No known reactivity to other proteins. The antibody specifically binds to the C-terminal epitope -T-E-T-S-Q-V-A-P-A- (COOH) of Rhodopsin. Proteins containing the epitope tag can be selectively bound to a Rho 1D4 affinity matrix and eluted using an excess of -T-E-T-S-Q-V-A-P-A- peptide under mild conditions.

Cross-Reactivity (Details): Species reactivity (tested):Vertebrates.

Characteristics: Synonyms: RHO, Opsin-2, OPN2

Purification: Protein G Chromatography

Immunogen: Bovine Rhodopsin.

Clone: 1D4

Isotype: IgG1

Application Details

Application Notes: ELISA. Western blot: 1/1000 (1)1 μg/mL was sufficient for detection of Rhodopsin in 10 μg of Rat eye lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1-2 μg per 100-500 μg of proteinImmunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 0.09 % Sodium Azide, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Rhodopsin

Target: Rhodopsin (RHO)

Alternative Name: Rhodopsin (RHO Products)

Synonyms: CSNBAD1 antibody, OPN2 antibody, RP4 antibody, RH1 antibody, Rh antibody, fi06d11 antibody, wu:fi06d11 antibody, zfo2 antibody, zfrho antibody, Noerg1 antibody, Opn2 antibody, Ops antibody, opn2 antibody, rh1 antibody, rp4 antibody, xrho antibody, PIGRHO1 antibody, ops antibody, RDP1 antibody, RHO antibody, rhodopsin antibody, rhodopsin L homeolog antibody, RHO antibody, Rho antibody, rho antibody, rho.L antibody

Target Type: Chemical

Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (Ref.2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).Synonyms: OPN2, Opsin-2, RHO

Molecular Weight: 48 kDa

Gene ID: 6010

UniProt: P08100

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction