PLA2R1 antibody (Internal Region)

Catalog No. ABIN487965

Product Details anti-PLA2R1 Antibody

Target: PLA2R1 (phospholipase A2 Receptor 1, 180kDa (PLA2R1))

Binding Specificity: Internal Region

Reactivity: Human, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This PLA2R1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: c-EKKKDTPKQH GT

Specificity: This antibody is expected to recognize all reported isoforms (NP_031392.3, NP_001007268.1, NP_001182570.1) of PLA2R1.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the Internal region of the protein sequence according to NP_031392.3, NP_001007268.1.

Application Details

Application Notes: Peptide ELISA: 1/64000. Western blot: Approx 140 kDa band observed in Human Ileum lysates (calculated MW of150 kDa according to NP_001007268.1). Recommended concentration: 1 - 3 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PLA2R1

Target: PLA2R1 (phospholipase A2 Receptor 1, 180kDa (PLA2R1))

Alternative Name: PLA2R1 / PLA2R (PLA2R1 Products)

Synonyms: Pla2-r antibody, Pla2g1br antibody, Pla2r antibody, CLEC13C antibody, PLA2-R antibody, PLA2G1R antibody, PLA2IR antibody, PLA2R antibody, phospholipase A2 receptor 1 antibody, PLA2R1 antibody, Pla2r1 antibody

Background: PLA2R is a receptor for secretory phospholipase A2 (sPLA2). It acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. It is also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, and selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. PLA2R may be involved in responses in proinflammatory cytokine productions during endotoxic shock. It also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B.Synonyms: 180 kDa secretory phospholipase A2 receptor, C-type lectin domain family 13 member C, CLEC13C, M-type receptor, PLA2-R, Secretory phospholipase A2 receptor

Gene ID: 22925

NCBI Accession: NP_001007268

UniProt: Q13018

Pathways: Positive Regulation of Response to DNA Damage Stimulus