UNC45B antibody (Internal Region)

Catalog No. ABIN488030

Product Details anti-UNC45B Antibody

Target: UNC45B (Unc-45 Homolog B (UNC45B))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This UNC45B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is expected to recognise both reported isoforms (NP_775259.1 and NP_001028748.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RSDKLRQKIFKER, from the internal region of the protein sequence according to NP_775259.1, NP_001028748.1.

Application Details

Application Notes: Peptide ELISA: Detection Limit Dilution: 1/2000. Western blot: 0.3-1 μg/mL. Approx 90 kDa band observed in Human Skeletal Muscle lysates(calculated MW of 100 kDa according to NP_775259.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C.

Target Details for UNC45B

Target: UNC45B (Unc-45 Homolog B (UNC45B))

Alternative Name: UNC45B (UNC45B Products)

Synonyms: MGC80931 antibody, cmya4 antibody, AA445617 antibody, Cmya4 antibody, D230041A13Rik antibody, Unc45 antibody, CMYA4 antibody, SMUNC45 antibody, UNC45 antibody, Unc-45B antibody, cb393 antibody, unc45r antibody, wu:fa05a03 antibody, wu:fa10f03 antibody, wu:fb37f02 antibody, wu:fb96f06 antibody, wu:fc10f04 antibody, unc-45 myosin chaperone B S homeolog antibody, unc-45 myosin chaperone B antibody, unc45b.S antibody, unc45b antibody, UNC45B antibody, Unc45b antibody

Background: UNC45B may play a role in sarcomere formation during muscle cell development. It may act as co-chaperone for HSP90 and may be necessary for proper folding of myosin. There are three named isoforms.Synonyms: CMYA4, Protein unc-45 homolog B, SMUNC45, UNC-45B, UNC45

Gene ID: 146862

NCBI Accession: NP_001028748

UniProt: Q8IWX7