TGFBI
Reactivity: Human, Mouse, Rat
WB, IHC, ELISA, ICC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Peptide ELISA: Detection Limit Dilution: 1/32000. Western Blot: 0.2-0.5 μg/mL. Approx 70 kDa band observed in Human, Mouse and RatKidney lysates (calculated MW of 74.0 kDa according to Human NP_000349.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
TGFBI, also known as BIGH3 and βig-h3, is an extracellular matrix protein induced by transforming growth factor (TGF)-beta 1. TGFBI protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of TGFBI were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain).Synonyms: Beta ig-h3, Kerato-epithelin, RGD-CAP, RGD-containing collagen-associated protein, Transforming growth factor-beta-induced protein ig-h3