Histone H1 antibody
| Antigen |
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| Synonyms |
H1c, H1.2, H1f2, His1a, H1var1, MGC107646, 0610008C09Rik, LOC692942, HISTONE H1, HON4 |
| Clonality |
Monoclonal (AE4) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunoprecipitation (IP)
Immunofluorescence (IF) (35), Western Blotting (WB) (27), Flow Cytometry (FACS) (18), ELISA (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunohistochemistry (IHC) (10), Immunoprecipitation (IP) (7), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN488120 |
| Quantity |
0.2 mg (1.0 mg/ml) |
| Price |
420.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: H1 Histone Family, H1F4, H1F5, Histone H1a, Histone H1b
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Immunogen
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Nuclei from myeloid leukamia biopsy cells
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Cross-Reactivity
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Human
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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AE4
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Description
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The histone proteins serve as a structural scaffold for the organisation of nuclear DNA intochromatin. In eucariotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 thatasseble to form an octamer round which the DNA is wrapped in a sequence dependantmanner. The resulting complex is known as a nucleosome and is the basic subunit ofchromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity,links these nucleosomes together resulting in the 30 nm chromatin fiber.
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Specificity
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This antibody detects specifically Histone H1. Species Reactivity: Tested: Human.
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Application Notes
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ELISA. Immunhistology on Frozen and Paraffin Sections: Acetone or PFA fixation is recommended for frozen sections. Positive Control Tissue: Tonsil. Immunoflourescence: Speckled staining is observed in the nucleus of normal andmalignant cells. Western blotting (1/100): in blots of Rat brain homogenate a duplet is observed at 27-29kDa consistent with Histone H1. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Purification
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Purified
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Buffer
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PBS containing 0.09% Sodium Azide as preservative.
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Storage
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Store the antibody undiluted at 2-8°C for one month or in aliquots at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Chromatin and Nuclear Signaling, DNA/RNA, Transcription Factors
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Restrictions
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For Research Use only
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Alternatives for antigen "Histone H1", type "Antibodies"
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Hosts
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Rabbit (28), Mouse (17), Chicken (4), Sheep (4)
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Reactivities
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Human (42), Cow (Bovine) (21), Mouse (Murine) (21), Rat (Rattus) (17), Dog (Canine) (16), Horse (Equine) (16), Rabbit (16), Pig (Porcine) (15)
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Applications
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Immunofluorescence (IF) (35), Western Blotting (WB) (27), Flow Cytometry (FACS) (18), ELISA (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunohistochemistry (IHC) (10), Immunoprecipitation (IP) (7), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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Acetyl-Lys25 (1), pThr17 (1)
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