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Chemokine (C-C Motif) Receptor 4 (CCR4) antibody

Antigen

Chemokine (C-C Motif) Receptor 4 (CCR4)
Synonyms CKR4, K5-5, CD194, CMKBR4, ChemR13, CC-CKR-4, MGC88293, HGCN:14099
Clonality Monoclonal (KH-4F5)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF)
Catalog no. ABIN488138
Quantity 0.1 mg  (2.0 mg/ml)
Price 370.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies back to top

Characteristics Synonyms: C-C chemokine receptor type 4, C-C CKR-4, CC-CKR-4, CCR-4, CMKBR4, K5-5
Alternative name CD194 / CCR4
Gene ID 1233
Swiss-Prot P51679
Immunogen Immunisation with Human CCR4 peptide. Remarks: Selection: Based on recognition of the complete native protein expressed ontransfected mammalian cells
Cross-Reactivity Human
Isotype IgG2a  (Matching secondary antibodies)
Clone KH-4F5
Description CCR4 (CC chemokine receptor 4) belongs to the rhodopsin family of G-protein-coupledreceptors. Chemokine receptors are membrane-bound molecules composed of7-transmembrane domains and are coupled to G-proteins (1,2). CCR4 binds thechemokines CCL17 (TARC) and CCL22 (MDC) and is highly expressed in most single-positiveCD4(+) thymocytes especially in TH2- and regulatory T-cells (3,4). It plays a central role in Tcell migration to the thymus, T cell maturation and education. CCR4 is often up-regulatedin inflammation and cause conformational changes that trigger intracellular signalingpathways (2,5).
Specificity Recognizes CCR4. Species Reactivity: Tested: Human. Add. Information: SDS-PAGE analysis: The antibody was purified by protein G affinity chromatography fromcell culture supernatants and verified by SDS-Page (Figure. 3).

Application Details
Alternatives Secondary Antibodies back to top

Application Notes cELISA: 1/200-1/400. Flow Cytometry: 1.2 µg/10e6 cells. Immunofluorescence: 1 µg/10e6 cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 2.0 mg/ml
Purification Purified
Buffer Phosphate buffered saline, pH 7.2
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area Chemokines, Receptors
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "Chemokine (C-C Motif) Receptor 4 (CCR4)", type "Antibodies"
Hosts Rabbit (8)
Reactivities Human (7), Mouse (Murine) (4), Rat (Rattus) (3), Dog (Canine) (2), Cow (Bovine) (1), Monkey (1)
Applications Western Blotting (WB) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunoprecipitation (IP) (1)