Structural Maintenance of Chromosomes 1A (SMC1A) (N-Term) antibody

Details for Product No. ABIN488213
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Antigen
Synonyms smc1a, smc1l1, zgc:76902, im:7158706, SMC1L1, SMC1L2, NV15570, CDLS2, DXS423E, SB1.8, SMC1, SMC1alpha, SMCB, 5830426I24Rik, SMC-1A, Sb1.8, Smc1, Smc1alpha, Smc1l1, Smcb, mKIAA0178, smc1aa, smc1, xSMC1
Epitope
N-Term
(49), (14), (12), (11), (7), (7), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(155), (83), (33), (26), (26), (26), (24), (12), (2), (2), (1), (1), (1)
Host
Mouse
(131), (25), (2), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(121), (55), (43), (33), (30), (15), (15), (8), (7), (3), (1)
Pubmed 1 reference available
Quantity 100 µL
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Catalog No. ABIN488213
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Immunogen Purified recombinant human SMC1A protein fragments expressed in E.coli.
Clone 8E6-A4-F3
Isotype IgG1
Specificity Subunit structure: Interacts with POLE. Interacts with SYCP2. Interacts with BRCA1. Found in a complex with CDCA5, SMC3 and RAD21, PDS5A/APRIN and PDS5B/SCC-112. Forms a heterodimer with SMC3 in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. In germ cell cohesin complexes, SMC1A is mutually exclusive with SMC1B. Interacts with BRCA1. Interacts with NDC80
Subcellular Location: Nucleus. Chromosome
Sensitivity This antibody detects endogenous levels SMC1A and does not cross-react with related proteins.
Purification Affinity purified
Purity > 90 %
Alternative Name SMC1A
Background Function: Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interac tion with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM BS1 branch and in the ATR/MSH2 branch of S-phase checkpoint..

Sequence similarities: Belongs to the SMC family. SMC1 subfamily

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation.
Synonyms: SMC1, SMCB, CDLS2, SB1.8, SMC1L1, DXS423E, KIAA0178, MGC138332, SMC1alpha, DKFZp686L19178
Molecular Weight 143 kDa
Gene ID 8243
NCBI Accession NM_006306
UniProt Q14683
Application Notes WB Dilution: 1:1000
FACS Dilution: 1:100
Restrictions For Research Use only
Format Liquid
Concentration 1. 000mg/ml
Buffer Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2 % sodium azide and 50 %, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Structural Maintenance of Chromosomes 1A (SMC1A) (N-Term) antibody SMC1A antibody at 1/200 dilution, Neuclear extracts of HeLa cell 40ug/Lane, Predicted band size: 150kDa, Observed band size: 150kDa
anti-Structural Maintenance of Chromosomes 1A (SMC1A) (N-Term) antibody (2) IHC of paraffin-embedded human colon using anti-SMC1A (N-terminus) diluted 1/500-1/1000.
Product cited in: Paech, Webb, Kuiper et al.: "Differential ligand activation of estrogen receptors ERalpha and ERbeta at AP1 sites." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1508-10, 1997 (PubMed).

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