Adenylate Kinase 2 antibody (AA 161-239)

Catalog No. ABIN4886446

Product Details anti-Adenylate Kinase 2 Antibody

Target: Adenylate Kinase 2 (AK2)

Binding Specificity: AA 161-239

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenylate Kinase 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Adenylate kinase 2, mitochondrial(AK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Adenylate kinase 2, mitochondrial(AK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: adenylate kinase 2
Protein Name: Adenylate kinase 2, mitochondrial

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human AK2 recombinant protein (Position: E161-I239). Human AK2 shares 93.7% and 93% amino acid (aa) sequence identity with mouse and rat AK2, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Adenylate Kinase 2

Target: Adenylate Kinase 2 (AK2)

Alternative Name: AK2 (AK2 Products)

Synonyms: ADK-2 antibody, BcDNA:SD09634 antibody, CG3140 antibody, Dak2 antibody, Dmel\\CG3140 antibody, anon-Dak2 antibody, adk2 antibody, NV10896 antibody, wu:fb34f05 antibody, wu:fj80e03 antibody, ADK2 antibody, AK 2 antibody, Ak-2 antibody, D4Ertd220e antibody, MXI22.8 antibody, MXI22_8 antibody, Adenylate kinase 2 antibody, adenylate kinase 2 antibody, adenylate kinase antibody, adenylate kinase 2 S homeolog antibody, Adenylate kinase family protein antibody, Adk2 antibody, AK2 antibody, ak2 antibody, LOC100123971 antibody, ak2.S antibody, Ak2 antibody, AT5G50370 antibody

Background: Adenylate kinase 2 is an enzyme is encoded in humans by the AK2 gene. The AK2 protein is found in the intermembrane space of the mitochondrion. Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates, this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.

Synonyms: Adenylate kinase 2 | ADK2 | AK 2 | ak2 | P54819

Gene ID: 204

UniProt: P54819

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process