DDAH2 antibody (C-Term)

Catalog No. ABIN4886561

Product Details anti-DDAH2 Antibody

Target: DDAH2 (Dimethylarginine Dimethylaminohydrolase 2 (DDAH2))

Binding Specificity: AA 190-224, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDAH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for N(G),N(G)-dimethylarginine dimethylaminohydrolase 2(DDAH2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: DAAQKAVRAM AVLTDHPYAS LTLPDDAAAD CLFLR

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for N(G),N(G)-dimethylarginine dimethylaminohydrolase 2(DDAH2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: dimethylarginine dimethylaminohydrolase 2
Protein Name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DDAH2 (190-224aa DAAQKAVRAMAVLTDHPYASLTLPDDAAADCLFLR), different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for DDAH2

Target: DDAH2 (Dimethylarginine Dimethylaminohydrolase 2 (DDAH2))

Alternative Name: DDAH2 (DDAH2 Products)

Synonyms: ddah2 antibody, MGC84290 antibody, g6a antibody, ddah antibody, ng30 antibody, ddahii antibody, MGC89103 antibody, DDAH2 antibody, DDAH antibody, DDAHII antibody, G6a antibody, NG30 antibody, 1110003M04Rik antibody, AU019324 antibody, AW413173 antibody, DDAH-2 antibody, Ddah antibody, dimethylarginine dimethylaminohydrolase 2 antibody, dimethylarginine dimethylaminohydrolase 2 L homeolog antibody, ddah2 antibody, ddah2.L antibody, DDAH2 antibody, Ddah2 antibody

Background: DDAH2 is known as dimethylarginine dimethylaminohydrolase 2 which is mapped to 6p21.3 by radiation hybrid and FISH analysis. This gene encodes a dimethylarginine dimethylaminohydrolase. DDAH2 functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants.

Synonyms: DDAH | DDAH II | DDAHII | DDAH2 | DDAH-2 | DDAH 2 | Dimethylargininase 2 | Dimethylargininase-2 | G6a | NG30 | Protein G6a | S phase protein | S-phase protein | O95865

Gene ID: 23564

UniProt: O95865