HNRNPF antibody (N-Term)

Catalog No. ABIN4886622

Product Details anti-HNRNPF Antibody

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Binding Specificity: AA 23-53, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Heterogeneous nuclear ribonucleoprotein F(HNRNPF) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: SVEDVQNFLS DCTIHDGAAG VHFIYTREGR Q

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Heterogeneous nuclear ribonucleoprotein F(HNRNPF) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: heterogeneous nuclear ribonucleoprotein F
Protein Name: Heterogeneous nuclear ribonucleoprotein F

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HnRNP F (23-53aa SVEDVQNFLSDCTIHDGAAGVHFIYTREGRQ), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for HNRNPF

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Alternative Name: HNRNPF (HNRNPF Products)

Synonyms: HNRPF antibody, OK/SW-cl.23 antibody, mcs94-1 antibody, HNRNPF antibody, Hnrpf antibody, 4833420I20Rik antibody, AA407306 antibody, QtsA-10715 antibody, hnRNP F antibody, heterogeneous nuclear ribonucleoprotein F antibody, HNRNPF antibody, Hnrnpf antibody

Background: Heterogeneous nuclear ribonucleoprotein F is a protein that in humans is encoded by the HNRNPF gene. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Synonyms: hnRNP F | hnRNPF | HNRPF | mcs94 1 | OK/SW-cl.23 | Ribonucleoprotein F | P52597

Gene ID: 3185

UniProt: P52597