Lactate Dehydrogenase A antibody (AA 2-106)

Catalog No. ABIN4886657

Product Details anti-Lactate Dehydrogenase A Antibody

Target: Lactate Dehydrogenase A (LDHA)

Binding Specificity: AA 2-106

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lactate Dehydrogenase A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for L-lactate dehydrogenase A chain(LDHA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for L-lactate dehydrogenase A chain(LDHA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: lactate dehydrogenase A
Protein Name: L-lactate dehydrogenase A chain

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human LDHA recombinant protein (Position: A2-R106). Human LDHA shares 94.3% amino acid (aa) sequence identity with both mouse and rat LDHA.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Lactate Dehydrogenase A

Target: Lactate Dehydrogenase A (LDHA)

Alternative Name: LDHA (LDHA Products)

Synonyms: GSD11 antibody, LDH1 antibody, LDHM antibody, LDH-M antibody, ldh-h antibody, ldha antibody, ldha-B antibody, ldhbb antibody, trg-5 antibody, Ldh1 antibody, Ldhm antibody, l7R2 antibody, gsd11 antibody, ldh-m antibody, ldh1 antibody, ldhm antibody, pig19 antibody, ldha1 antibody, ldhab antibody, LDH-A antibody, LDHC antibody, lactate dehydrogenase A antibody, lactate dehydrogenase B L homeolog antibody, lactate dehydrogenase A4 antibody, lactate dehydrogenase A L homeolog antibody, L-lactate dehydrogenase A chain antibody, LDHA antibody, ldhb.L antibody, Ldha antibody, ldha antibody, ldha.L antibody, LOC100713875 antibody

Background: Lactate dehydrogenase A, also known as LDHA, is an enzyme which in humans is encoded by the LDHA gene. The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

Synonyms: GSD11 | l7R2 | LDH A | LDH-A | LDH M | LDH-M | LDH1 | ldha | LDHM | PIG19 | P00338

Gene ID: 3939

UniProt: P00338

Pathways: Warburg Effect