LDHB antibody (AA 237-334)

Catalog No. ABIN4886658

Product Details anti-LDHB Antibody

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Binding Specificity: AA 237-334

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for L-lactate dehydrogenase B chain(LDHB) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for L-lactate dehydrogenase B chain(LDHB) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: lactate dehydrogenase B
Protein Name: L-lactate dehydrogenase B chain

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human LDHB recombinant protein (Position: E237-L334). Human LDHB shares 98% amino acid (aa) sequence identity with both mouse and rat LDHB.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for LDHB

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Alternative Name: LDHB (LDHB Products)

Synonyms: LDH-B antibody, LDH-H antibody, LDHBD antibody, TRG-5 antibody, AI790582 antibody, H-Ldh antibody, Ldh-2 antibody, Ldh2 antibody, ldh-h antibody, ldh2 antibody, ldhb antibody, ldhba antibody, trg-5 antibody, LDHB antibody, LDH-B-A antibody, wu:fa16d07 antibody, wu:fa20c10 antibody, wu:fa96a08 antibody, LDH-B-B antibody, zgc:92882 antibody, lactate dehydrogenase B antibody, lactate dehydrogenase B S homeolog antibody, lactate dehydrogenase Ba antibody, lactate dehydrogenase Bb antibody, LDHB antibody, Ldhb antibody, ldhb.S antibody, ldhb antibody, ldhba antibody, ldhbb antibody

Background: The LDHB gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.

Synonyms: HEL S 281 | LDHB | LDH-B | LDH B | LDHH | LDH-H | LDH H | LDHBD | TRG-5 | TRG5 | P07195

Gene ID: 3945

UniProt: P07195

Pathways: Warburg Effect